Our LB Broth (Miller) is a pre-prepared, sterile liquid culture medium formulated for robust bacterial growth. Commonly used for culturing Escherichia coli and other non-fastidious microorganisms, this ready-to-use broth eliminates the need for weighing, mixing, or autoclaving—just open and inoculate.
Composition (per liter):
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Tryptone – 10 g
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Yeast Extract – 5 g
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Sodium Chloride (NaCl) – 10 g
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pH ~7.0 ± 0.2 at 25°C
This is the Miller formulation, featuring a higher NaCl concentration (10 g/L) compared to LB Lennox, making it ideal for most cloning and plasmid prep workflows.
Storage:
Store refrigerated or at room temperature. Keep sealed when not in use to maintain sterility and shelf life.