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Laboratory prepared • Ready to use

LB Broth (Miller) - Sterile, Ready-to-Use Liquid Media

LB Broth (Miller) - Sterile, Ready-to-Use Liquid Media

$6.95 USD
$6.95 USD
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Size
  • Laboratory prepared
  • Ready to use
  • Direct lab support
Sterile, ready-to-use Miller-formulation LB broth for routine cultivation of Escherichia coli and other non-fastidious bacteria.

Laboratory & recurring orders

Need the same product regularly or in larger quantities? Contact our laboratory team to discuss a recurring or larger order.

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Technical details

Applications

Routine bacterial cultivation; cloning workflows; plasmid preparation; general molecular-biology workflows.

Formulation

Per liter: tryptone 10 g; yeast extract 5 g; sodium chloride 10 g. Finished-product release pH: 7.0 ± 0.2 at 25°C.

Storage

Unopened: Store at 4–30°C. Ambient storage within this range is acceptable for shorter-term use; refrigeration is recommended for longer-term storage. After opening: Refrigerate and use appropriate aseptic handling. Brief excursions above 30°C during normal shipping are acceptable; avoid prolonged excessive heat and temperatures low enough to cause freezing.

Shelf life

Unopened: 2 years. After opening: Up to 6 months when refrigerated and handled appropriately using aseptic technique.

Quality control

Growth tested with suitable microorganisms to confirm expected growth. After filling, bottles are observed for a minimum of 48 hours for evidence of unintended microbial growth as a contamination/sterility QC check. Finished-product pH, appearance, and fill volume are verified. Each finished unit receives a lot number for traceability. Media are sterilized by autoclaving; autoclave sterilization is monitored using biological/spore indicators and temperature-sensitive chemical indicator tape. Components requiring separate treatment are filter sterilized.

LB Broth (Miller) is supplied sterile and ready to use for routine cultivation of Escherichia coli and other non-fastidious bacteria.

Select the appropriate volume, inoculate using aseptic technique, and incubate under the conditions required by your organism and protocol.